ALDEFLUOR™ kit for the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH. ALDEFLUOR&#; Kit from. PRODUCT DESCRIPTION. ALDEFLUOR™ is a reagent kit that is used to identify human cells that express high levels of the enzyme aldehyde dehydrogenase. Here is a quick guide to setting up your FACS analysis with ALDEFLUOR: We’ ve used the Aldefluor kit extensively for both cell lines and for freshly dissociated .
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For optimal assay performance, lyse the erythrocytes by treating the samples with ammonium chloride.
ALDEFLUOR™ Kit | STEMCELL Technologies
Cancer Res 69 Storms RW aldef,uor al. This product is designed for use in the following research area s as part of the highlighted workflow stage s. In the DEAB-treated control, fluorescence will reflect the size of the intracellular substrate pool. Man CH et al.
Tissue Dissociation and Isolation. Aldefluod Cells 27 7: However, the as-yet poorly characterized contribution aldeflhor host responses during tumour growth and metastatic progression represents a limitation to exploiting this approach. Prostate Epithelial Cell Research. Is it acceptable for the staining reaction to exceed 30 minutes? Explore these workflows to learn more about the other products we offer to support each research area.
Buijs JT et al. We suggest a range of concentrations from 5-fold less to fold more than the standard concentration.
Eur J Cancer 50 1: Prostate Cells Yao M et al. Azzam DJ et al.
Increasing cell concentrations greater than 5-fold the recommended concentration will decrease assay signal and thereby decrease discrimination of the ALDHbr population. Le Magnen C et al. To stain large number of cells it may be better to increase the sample and reagent volume. High ALDH expression has alvefluor reported for normal and cancer precursor cells of various lineages, including hematopoietic, mammary, endothelial, mesenchymal, and neural.
Brit J Cancer Data and Publications Data. Viale A et al. Am Heart J 2: Neutrophils have kih fundamental role in inflammatory responses and their contribution to tumorigenesis is still controversial. In this article, we demonstrate that dietary fiber and short chain fatty acids SCFAs induced the expression of the vitamin A-converting enzyme RALDH1 in intestinal epithelial cells in vivo and in vitro, respectively.
Christ O et al. Other ALDH inhibitors can be used as appropriate for the enzyme isoform expressed. Cancer Res 73 5: J Exp Med Povsic TJ et al.
Silva IA et al. Endothelial Cells Povsic TJ et al.
Yamamoto M et al. Rasheed Z et al. Clin Cancer Res 19 Our iPSC-derived MSCs were readily expandable, but still underwent senescence after prolonged culture and did not form teratomas. Kir Malignancies Boucher K et al. Brit J Cancer 7: Our results reveal the efficacy of using targeted therapy against a specific tumour microenvironment component and indicate that neutrophil Alox5 inhibition may limit metastatic progression.
Gentry T et al. Yes, but full staining will take at least 3 – 4 hours.
J Immunol 1: Atkinson RL et al.