ASTM E Antimicrobial Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents under Dynamic Contact Conditions. The test.
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It has been developed for routine quality control and screening tests and is astn to evaluate substrate bound antimicrobials. These difficulties include ensuring contact of inoculum to treated surface as in AATCCflexibility of retrieval at different contact times, use of inappropriately applied static conditions as in AATCCsensitivity, and reproducibility. No regulated reduction limits currently exist for general antimicrobial claims made using this method.
This dynamic shake flask test was developed for routine quality control and screening tests in order to overcome difficulties in using classical antimicrobial test methods to evaluate substrate-bound antimicrobials.
The antimicrobial activity of a substrate-bound, non-leaching antimicrobial agent is dependent upon direct contact of microbes with the active chemical agent. The flask is placed onto a wrist-action shaker and shaken for a desired astmm time, typically 1 hour. The test quantitatively evaluates the effectiveness of a sample treated with a non-leaching antimicrobial agent by shaking in an organism suspension.
These cookies collect information about how you interact with our website and allow us to remember you. The ASTM E Standard Test Method is used to evaluate the antimicrobial activity of substrate-bound, non-leaching, antimicrobial-treated samples under dynamic contact conditions.
Following exposure, a sample of the test 2e149 suspension is removed quantitatively assayed for survivors. These difficulties include ensuring contact of inoculum to treated surface as in AATCCflexibility of retrieval at different contact times, use of inappropriately applied static conditions as in AATCCsensitivity, and reproducibility. This test method standardizes both the growth conditions of the challenge species and substrate contact times to reduce the variability associated with growth phase of the microorganism.
Work Item s – proposed revisions of this standard.
A single cookie will be used in your browser to remember your preference not to be tracked. It is for this reason that the use of the term leaching throughout this document is limited to only the testing conditions described herein. Summary of Test Method: No other units of satm are included in this standard.
In this method, treated test samples are placed in a laboratory flask containing a dilute suspension of test organism, commonly Escherichia coli. This standard does not purport to address all of the safety concerns, if any, associated with its use. This dynamic shake flask test was developed for routine quality control astn screening tests in order to overcome f2149 in using classical antimicrobial test methods to evaluate substrate-bound antimicrobials.
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If an antimicrobial agent that is shown to be removed from the surface by Section 13 is utilized in this test methodology, controls must be included such that appropriate neutralization steps are including during recovery and enumeration. The test determines the antimicrobial activity of a treated specimen by shaking samples of surface-bound materials in a concentrated bacterial suspension for a one hour contact time. Surface antimicrobial activity is determined by comparing results from the test sample to controls run simultaneously.
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Summary of Test In this method, treated test samples are placed in a laboratory flask containing a dilute suspension of test organism, commonly Escherichia coli. Facebook Linkedin Instagram Twitter Google.
Presence of residual antimicrobial activity indicates the presence ast a leaching type of antimicrobial agent. This method is generally used to substantiate antimicrobial properties of treated specimens for non-public health related claims. This test ensures good contact between bacteria and ashm treated fibre by constant agitation of the test specimen in a bacterial suspension during the test period 1hour.
This test method is only intended to determine efficacy as described in 4. Log in Log out Edit. The resulting plates are incubated, the number of survivors is enumerated and a percent reduction is determined for the test flask as compared to the untreated control suspension.
Organisms typically used in this method: The number of viable organisms from the suspension is determined and the percent reduction is calculated by comparing retrievals from appropriate controls. All the samples are shaken in dynamic shake flasks in a wrist action shaker to ensure good contact between the bacteria and the treated fibre, fabric, or other substrate by constant agitation of the test specimen in a bacterial suspension during the test period.
ASTM E – Microbe Investigations (MIS)
One-hour contact time in a buffer solution allows for metabolic stasis in the population. For example, water soluble antimicrobials will be prone to removal from the test surface using the method described in Section 13 but insoluble compounds will not. Referenced Documents purchase separately The documents listed below are referenced within the subject standard but are not provided as part of the standard. Link to Active This link will always route to the current Active version of the standard.
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Materials evaluated by this method may be comprised of fabric, paper, powder or other solid materials. Historical Version s – view previous versions of standard. Immobilized antimicrobial agents, such as surface bonded materials, are not free to diffuse into their environment under normal conditions of use.
This test method ensures good contact between the bacteria and the treated fiber, fabric, or other substrate, by constant agitation of the test specimen in a challenge suspension during the test period.