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Always contact us first. Antibodies and their sources were as follows: As shown in Fig. Thus, in vitro auto-phosphorylation of c-Src serves dx a measure of its auto-activation. Same day dispatch if ordered before 3: ZhaoLan Tang, Kenneth S. Using either Triton-based methods 121314151617 or de methods 181920it has been demonstrated that caveolin co-purifies with cytoplasmic lipid-modified signaling molecules.

Classifications Cell Biology and Metabolism.

One aliquot was subjected to an immune complex kinase assay see below. We cannot process a return if you do not email us.

Contact the seller – opens in a new window or tab and request a postage method to your location. Materials The cDNA for canine caveolin was as 21990 previously Get the item you ordered or your money back. These two gene products differ primarily in their extreme C-terminal regions 3852 No additional import charges on delivery.

Besides interacting with Src family kinases, this cytosolic caveolin domain residues has the following unique features. See all condition definitions – opens in a new window or tab Note that both c-Src and v-Src migrate at 60 kDa as indicated.


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In addition, double labeling of T cells co-transfected with c-Src and caveolin revealed significant co-localization of these two distinct gene products Fig. Learn more – opens in a new window or tab.

Regarding allosteric inhibitors, they could act by xs the equilibrium in favor of the inactive conformation, perhaps by masking Tyr from kinases or Tyr from phosphatases 37 Effect of caveolin peptides on the auto-phosphoryation of c-Src and Fyn tyrosine kinases. This item doesn’t fit this. After expression, c-Src kinase was isolated by immunoprecipitation and subjected to the immune complex kinase assay Fig.

This differential effect was also observed when these three peptides were used to evaluate their effects on the functional activity of hetero-trimeric G-proteins. In contrast, recombinant overexpression of wild-type c-Src does not result in efficient cell transformation or increased tyrosine phosphorylation, indicating that the tyrosine kinase activity of c-Src is normally repressed 37 Recently, two caveolin-related proteins caveolin-2 and caveolin-3 have been identified and cloned; caveolin has been retermed caveolin Untagged caveolin and c-Src were co-expressed in T cells by co-transfection using a modified calcium phosphate precipitation procedure as described previously Description Postage and payments.

The effects of peptide 1 A and peptide 2 B are shown. For example, Shoelson and colleagues have shown that high concentrations of peptide substrates inhibited the autophosphorylation of c-Src kinase Two general classes of tyrosine kinases have been defined: Caveolae are plasma membrane specializations present in most cell types.

Add to Watch list Watching. This listing is for the part or sub-assembly number shown below only. For additional information, see the Global Shipping Programme terms and conditions – opens in a new window or tab This amount includes applicable customs duties, taxes, brokerage and other fees.


After 3 days of incubation, cells and culture supernatants were removed and centrifuged at 1, rpm for 10 min. The numbers at end points reflect their exact amino acid position within caveolin.

21990 Email this article to a friend Alert me when this article is cited Alert me if a correction is posted Alert me when eletters are published Similar articles in this journal Similar articles in Web of 2990 Similar articles in PubMed Download to citation 2910 Request Permissions.

Note that peptides 3 and 4 correspond to the N-terminal and C-terminal halves of peptide 2, respectively. Watch list is full. Journal of Lipid Research.

First, it is required to form multivalent homo-oligomers of caveolin. Related Content Load related web page information. Caveolin Residues Functionally Inhibit the Auto-activation of c-Src and Fyn Tyrosine Kinases To examine the functional consequences of the interaction of caveolin with Src tyrosine kinases, we generated a panel of caveolin peptides that span the region of caveolin. Caveolin peptides were examined for their effect on the autophosphorylation of purified recombinant Fyn kinase in vitro.

Thus, caveolin may organize the formation of caveolae microdomains and orchestrate caveolae-related signaling events. Accepted, Eligibility for PayPal Credit is determined at checkout.