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Membrane is a semiperiable tissue. Now days, the Research membrane filtration process is mostly done, but the Journal article Jurnal Biofisika • Jurnal Biofisika Vol 8, No 1 (): Jurnal Biofisika. SIFAT LISTRIK MEMBRAN SELULOSA ASETAT – TITANIUM DIOKSIDA. Juansah, Jajang. Chemistry: A European Journal, (accepted article, in press). . Membrane insertion of the BAX core, but not latch domain, drives apoptotic pore formation.

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Shin JH, et al. Biochemical evidence for lead and mercury induced transbilayer movement of phospholipids mediated by human phospholipid scramblase 1. British journal of haematology. Journal of Biological Chemistry.

The mechanism of lead-dependent cell lysis was explored next. Fluorescence-activated cell sorting analysis Fluorescence-activated cell sorting FACS analysis was performed as described previously Retention of water and potassium by erythrocytes prevents calcium-induced membrane rigidity. The former was labeled with an antiCer specific antibody ALX— as primary, Alexa Fluor as secondarywhile the latter was identified through the ability of PS to bind Annexin V.

Posada IM, et al.

Pb(II) Induces Scramblase Activation and Ceramide-Domain Generation in Red Blood Cells

Whole blood was obtained from informed healthy volunteers by venipuncture. Coverslips were mounted on glass slides. This supports the flow of information summarized in Fig.


Table 3 Inhibition of Pb II -induced hemolyisis. The influence of valinomycin induced membrane potential on erythrocyte shape. Increased intracellular calcium levels can activate scramblase, which in turn causes PS exposure We had also observed that mechanical stress elicited a neutral sphingomyelinase activity in erythrocytes Cells treated with the neutral SMase inhibitor GW showed much decreased ceramide contents.

Functions of ceramide in coordinating cellular responses to stress. Images are a combination of transmitted light and fluorescence confocal microscopy, where red dots indicate ceramide-enriched zones detected by the antiCer- bioifsika Alexa Fluor as secondary antibody.

Asymmetric addition of ceramides but not dihydroceramides promotes transbilayer flip-flop lipid motion in membranes. Open in a separate window. Hasna Ahyayauch1, 3, 4 Aritz B. This is particularly clear in Fig.

This process can be stopped at this point using clotrimazole. These results are in good agreement with those by Kempe, et al.

Pb(II) Induces Scramblase Activation and Ceramide-Domain Generation in Red Blood Cells

In the next stage spherocytes are formed. Calcium binding studies of peptides of human phospholipid scramblases 1 to 4 suggest that scramblases are new class of calcium binding proteins in the cell.

Kempe DS, et al. Isolation of RBC Whole blood was obtained from informed healthy volunteers by venipuncture.


This can be due to the lack of three-dimensional imaging in these samples. Differentiation-dependent expression of phosphatidylserine in mammalian plasma membranes: Received Sep 1; Accepted Apr In particular, PS translocation from the inner to the outer leaflet of the plasma membrane is critical for recognition and clearance of apoptotic cells by macrophages 52 Phosphatidylserine exposure and red cell viability in red cell aging and in hemolytic anemia.


Lead-induced phosphatidylserine translocation in scramblase-transfected CHO cells. SMase inhibition by GW does not inhibit echinocytosis Fig.

Jurnal Biofisika

Mechanisms and significance of eryptosis. The Journal of clinical investigation. Ceramide-enriched membrane domains in red blood cells and the mechanism of sphingomyelinase-induced hot-cold hemolysis. Biochimica et biophysica acta. Moreover, data from this and other laboratories suggest that a direct cholesterol-ceramide interaction may be taking place at high concentrations of both lipids 27 Intracellular free calcium was measured with Fura-2AM.

Bratosin D, et al. Programmed cell death in mature erythrocytes: Guri Y, et al. Confocal fluorescence microscopy studies were carried out with the aim of detecting and localizing ceramide and externalized PS.

All other reagents and solvents for assay were of analytical reagent grade and were purchased from Sigma-Aldrich.

Mandal S, et al.